Cell Stimulation Cocktail (plus protein transport inhibitor) (500X) is phorbol 12-myristate 13-acetate (PMA), ionomycin, Brefeldin A and monensin. Treatment with PMA and ionomycin is sufficient to induce activation and cytokine production in many cell types. Brefeldin A and monensin cause accumulation of secreted proteins in the endoplasmic reticulum and Golgi apparatus. Therefore, Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) can be used for the induction and subsequent induction of cytokines and other secreted proteins in in vitro cultured and ex vivo cells. Intracellular detection.

For unstimulated and solvent controls, see Protein Transport Inhibitor Cocktail (500X) (Cat. No. 00-4980).

Reported Applications
Flow cytometry analysis, functional analysis, cell culture