Thermo Fisher 11966025 500 mL DMEM, does not contain glucose
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11966025 500 mLDMEM without glucose
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DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that can be used to support many types of mammalian cells grow. Cells that have been successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells, and cell lines such as HeLa, 293, Cos-7, and PC-12. For a wide range of cell culture applications, DMEM modified media with various components are provided. Use Medium Selection Tool Find the right recipe.
This DMEM is improved as follows:
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Full formulation.
Using DMEM
DMEM differs from other media in that it contains 4 times the concentration of amino acids and vitamins equivalent to the original Eagle Minimum Essential Medium. DMEM is initially formulated with low concentrations of glucose (1 g/L) and sodium pyruvate, but is often used in high glucose environments with/without sodium pyruvate. DMEM contains no proteins, lipids or growth factors. Therefore, DMEM needs to be supplemented with nutrients, usually 10% Fetal bovine serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP Production and Quality System
To ensure the stability of the supply chain, we produce DMEM simultaneously in two independent factories in Grand Island, New York and Scotland, UK. Both factories comply with cGMP production requirements, are ISO 13485 certified, and are FDA-registered medical device manufacturers.
This DMEM is improved as follows:
| Includes | Does not contain td> |
| •L-Glutamine | •Glucose |
| •Phenol Red | •Sodium Pyruvate |
| • HEPES |
Full formulation.
Using DMEM
DMEM differs from other media in that it contains 4 times the concentration of amino acids and vitamins equivalent to the original Eagle Minimum Essential Medium. DMEM is initially formulated with low concentrations of glucose (1 g/L) and sodium pyruvate, but is often used in high glucose environments with/without sodium pyruvate. DMEM contains no proteins, lipids or growth factors. Therefore, DMEM needs to be supplemented with nutrients, usually 10% Fetal bovine serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP Production and Quality System
To ensure the stability of the supply chain, we produce DMEM simultaneously in two independent factories in Grand Island, New York and Scotland, UK. Both factories comply with cGMP production requirements, are ISO 13485 certified, and are FDA-registered medical device manufacturers.
For Research Use Only. Not for use in diagnostic procedures.
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