Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium used to support many types of mammalian cells grow. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glia, HUVECs, smooth muscle cells, and cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modified media suitable for a wide range of cell culture applications. Use
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This DMEM is improved as follows:
| Includes | Does not contain | < /tr>
| •High sugar | •Sodium pyruvate |
| •L-Glutamine | •HEPES: |
A complete Recipe.
DMEM differs from other media in that it contains 4 times the concentration of amino acids and vitamins equivalent to the original Eagle Minimum Essential Medium. DMEM is initially formulated with low concentrations of glucose (1 g/L) and sodium pyruvate, but is often used in high glucose environments with/without sodium pyruvate.
cGMP Manufacturing and Quality Systems
Gibco™ DMEM is manufactured in a cGMP compliant facility in Paisley, Scotland, UK. The facility is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure supply chain stability, we also offer a comparable Gibco™ DMEM product (12100-061) manufactured at our Grand Island facility. The facility is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.
DMEM does not contain proteins, lipids or growth factors. Therefore, DMEM needs to be supplemented with nutrients, usually 10% Fetal bovine serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Gibco™ cell culture media in powder form requires supplementing sodium bicarbonate, adjusting pH, and filtering (for details, see protocol). 
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