Click-iT EdU Cell Proliferation Imaging Kit is an alternative to traditional cell proliferation assays for fluorescence microscopy The application has been optimized. In this assay, the modified thymidine analog EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with bright, photostable Alexa Fluor™ dyes in a fast, highly specific click reaction. Thanks to the gentle click protocol, this fluorescent labeling of proliferating cells is highly accurate and compatible with antibody methods.

•Simple—get the job done in less time every time
•Efficient—no denaturation steps or harsh handling required
•Rich detection results—better preservation of cell morphology, Antigen structure and DNA integrity
• Consistent—not dependent on variable antibody batches for detection

Accurate cell proliferation detection method is based on the incorporation of nucleoside analogues into newly synthesized DNA and measurement, of which bromodeoxyuridine (BrdU) is a commonly used analogue. BrdU-labeled DNA is quantified using anti-BrdU antibodies after denaturation of DNA by harsh treatments (HCl, heat, or enzymes) and exposure to BrdU molecules. This step is time-consuming and difficult to perform consistently. Harsh handling can also adversely affect sample integrity and quality, making co-staining with other antibodies challenging.

Excellent Proliferation Methodology
The Click-iT EdU Cell Proliferation Imaging Kit provides a cell proliferation assay superior to the BrdU assay. EdU (5-ethyl-2'-deoxyuridine) is a nucleoside analog of deoxythymine that is incorporated into DNA during active DNA synthesis. With Click-iT™ EdU, gentle fixation and detergent permeabilization are sufficient to allow small molecule-based Click-iT™ EdU detection reagents to gain access to the pathways for incorporation into DNA. As a result, the Click-iT™ EdU Imaging Kit is not only easy to use, but also more accurate and compatible with cell cycle analysis and other intracellular or extracellular target components for satisfying and enriched detection results.

This kit is optimized for fluorescence microscopy applications; if you require a kit designed for high-content imaging, fluorescence microplate detection, or flow cytometric analysis platforms, please visit Click-iT on our website ™ Technical Section.

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Notes:
By feeding The Click-iT™ Assay Kit can be used with cells in culture or in vivo after administration of EdU via injection or injection.
The Click-iT™ Assay Kit can be used with BrdU in double-pulse experiments by using an anti-BrdU (clone MoBu-1) antibody that does not cross-react with EdU.
Click-iT™ technology is compatible with immunohistochemistry, immunocytochemistry, and fluorescent dyes that are fixation-resistant or designed for labeling of fixed cells.