Thermo Fisher C34554 180 reactions CellTrace CFSECell Proliferation Kit (for flow cytometric analysis)
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C34554 180 reactionsCellTrace CFSE Cell Proliferation Kit (for flow cytometric analysis)

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CellTrace™ CFSE Cell Proliferation Kit is suitable for labeling cells in vitro and in vivo and uses a dye dilution method to track multiple cells through flow cytometry analysis. generation cells.

•Excellent performance—bright single-peak staining enables visualization of cells over multiple generations
•Long signal stability—remains in cells for days after staining
•Nontoxic—staining does not Adversely affects cell health
•Simple and reliable staining protocol

Viewfor flow Selection guide for all CellTrace™ Cell Proliferation Kits for cell analysis.

Excellent fluorescence staining
Successful proliferation analysis using the dye dilution method (see image below) requires extremely bright dyes to differentiate fluorescence after several cell divisions Labeled cells and autofluorescence. CellTrace™ CFSE dye produces high-intensity fluorescent staining, allowing visualization of eight or more generations of proliferating cells before the cell's intrinsic autofluorescence masks the labeled fluorescent signal. The staining results are consistent and uniform, with little fluorescence variation between cells in the population, and cell generations can be clearly differentiated without the need for complex modeling software.

Long signal retention
Unlike stains that label cellular lipid membranes, CellTrace™ CFSE stains readily cross the plasma membrane and covalently bind to intracellular materials Combined, the fluorescent dye remains well retained and relatively stable, providing a consistent fluorescent signal even when left in a cell culture environment for several days.

Non-toxic
CellTrace™ CFSE dye can covalently bind to all free amines on the cell surface and within cells, with less cytotoxicity and no impact on cell proliferation or cell biology. Extremely small. Researchers have used CFSE SE labeling to observe the proliferation of transplantable hematopoietic stem cells in vitro after being stimulated by a combination of growth factors.

Simple and reliable staining protocol
CellTrace™ CFSE Cell Proliferation Kit contains dye powder in a convenient single-use vial for small-scale experiments without the need to prepare excess dye. Before use, prepare a stock solution by dissolving the contents of the vial in anhydrous DMSO. To stain cells in 1 mL of protein-free medium, typically use 1 µL of stock solution. Cells were stained for 20 minutes at room temperature with gentle stirring during staining. This is followed by a brief wash with complete medium and quenching of any remaining dye in the solution.
For Research Use Only. Not for use in diagnostic procedures.