The CyQUANT® Cell Proliferation Assay is a highly sensitive fluorescent assay for quantifying cells and assessing cell proliferation and cytotoxicity. For a cell proliferation assay that is more compatible with high-throughput screening, please see our
CyQUANT® NF Cell Proliferation Assay a> or CyQUANT® direct detection.
Using the CyQUANT® Cell Proliferation Assay you can:
• Measure DNA content to directly quantify 50 to 50,000 cells/well without relying on metabolic activity
• Use well plate-based Simple assay to quickly compare multiple samples
• Freeze and store time course samples for later analysis
Directly measure DNA contentCyQUANT® Cell Proliferation Assay Kit The main ingredient is CyQUANT® GR, a proprietary dye that exhibits strong fluorescence enhancement when combined with nucleic acids. Using this dye, you can measure the DNA content of your sample and compare it to standards, allowing you to directly quantify entire cell populations over a broad linear detection range. This method provides greater accuracy than metabolism-based cell proliferation or cytotoxicity assays, which may be affected by cellular changes independent of cell number.
Quickly compare multiple samplesThe CyQUANT® Cell Proliferation Assay requires minimal hands-on time. Simply remove the culture medium, freeze and lyse the cells, and read the fluorescence using a standard microplate reader with a fluorescein filter.
Storing samples for later analysisBecause the cells are frozen, samples can be stored for up to 4 weeks and run in batches to allow direct comparison of samples taken at different time points.
Kit ComponentsThe kit contains sufficient CyQUANT® GR dye and cell lysis buffer for 1,000 assays in a volume suitable for microplate fluorescence detection. The kit also contains phage lambda DNA for use as a reference standard for assay calibration.
For Research Use Only. Not for use in diagnostic procedures.