Thermo Fisher F548L 500 reactions Phusion Flashhigh fidelity PCRmaster mix

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable lab time. Ready-to-use 2X Master Mix maintains reaction fidelity and yield when using extremely short PCR protocols. Additionally, users only need to add template and primers, minimizing pipetting steps.

Unique master mix composition allows for extremely short cycle protocols - 15 seconds per kb or less - for both low and high complexity DNA templates. This master mix uses Phusion Flash II DNA Polymerase, a modified proofreading DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase.

Features
• Extremely high speed: 15 s/kb or less stretch time
• Warm start modification allows "zero time reactivation"
• Accuracy: Proofreading DNA polymerase with 25X Taq fidelity
• Get high yields in less time

Applications

• Fast PCR
• High-fidelity PCR
• Difficult (high GC content) templates
• Obtaining sequencing templates
• Multiplex PCR
• Long PCR
• Cloning
• Lure Variable
• Microarray

Using Phusion DNA Polymerase
Annealing temperature design rules for Phusion DNA polymerase and many common DNA polymerases (such as Taq DNA polymerase) no the same. For best results, use www.thermofisher.cn/tmcalculator.