Thermo Fisher FD1314 20 reactions FastDigest BplI

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N13

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Thermo Scientific FastDigest BplI restriction enzyme recognizes the ^(8/13)GAG(N)5CTC(13/8)^ site and uses the universal FastDigest Buffer cleaves best within 5–15 minutes at 37°C.

Thermo Scientific FastDigest BplI is one of a series of high-end fast restriction enzymes that are 100% active in both Universal FastDigest and FastDigest Green Reaction Buffers.

Universal buffer can quickly complete DNA single, double or multiple enzyme digestion in 5–15 minutes without the need for buffer changes or subsequent DNA purification steps. For a table of enzymatic activities, heat inactivation, and incubation times for this and other FastDigest restriction enzymes, see Reaction conditions for FastDigest enzyme< /a>. DNA modifying enzymes (such as Klenow fragment, T4 DNA Ligase, alkaline phosphatase and T4 DNA Polymerase) are 100% active in FastDigest buffer. Therefore, enzymes used in downstream applications can be added directly to the FastDigest reaction mixture.

For convenience, FastDigest Green Buffer includes 1 density reagent and 2 tracking dyes for loading digestion reaction products directly onto the gel.

Shorter incubation times and optimized universal FastDigest buffer composition eliminate star activity effects.

Features

•100% activity of all FastDigest enzymes in universal buffers
•100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Load directly onto gel
• No star activity
• 176 FastDigest enzymes available

Applications< /b>

•Molecular cloning
•Restriction site mapping
•Genotyping
•Southern blot
•Restriction fragment length polymorphism (RFLP)
• SNP Analysis

Remarks:FastDigest BplI requires only Mg²⁺ to exert its activity, but is stimulated by SAM. 0.05 mM S-adenosylmethionine increases FastDigest BplI activity more than 100-fold. FastDigest BplI still has difficulty digesting some substrates completely.

FastDigest Green Buffer provides the same high performance as colorless FastDigest Buffer in DNA digestion reactions and downstream applications. For applications requiring product analysis by fluorescence excitation (eg determination of concentration under UV light), the use of colorless FastDigest buffer is recommended.

See product specifications for information on methylation sensitivity.