Normal mitochondrial membranes maintain an electrical potential difference between the inside and outside of the organelle, called membrane potential. Tetramethylrhodamine, methyl ester (TMRM) is a cell-permeable dye that can accumulate in active mitochondria with intact membrane potential. If the cell's mitochondria are normal and functioning, they emit a fluorescent signal. Once the mitochondrial membrane potential is lost, TMRM no longer accumulates and the fluorescence signal becomes weaker or disappears. TMRM signals can be detected by fluorescence microscopy, flow cytometry, cell sorting, high-throughput screening, and high-content analysis.
•For live cell dynamic studies of mitochondrial membrane potential (unfixed)
• RFP/TRITC filters available (absorption peak: 548 nm, emission peak: 574 nm)
• Available Multicolor detection with green, crimson, violet and blue emitting dyes such as GFP, DAPI, Hoechst, HCS LipidTOX and LysoTracker Deep Red
For Research Use Only. Not for use in diagnostic procedures.