Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO™ Expression Vector (Figure 1) enables you to quickly clone your gene and It was fused to the widely used and well-characterized Aequorea victoria fluorescent protein (FP) (1, 2). These efficient TOPO™ cloning vectors contain emerald fluorescent protein (EmGFP) or yellow fluorescent protein (YFP) for simple, non-invasive detection of recombinant proteins (Figure 2). Both FPs have been humanized for excellent mammalian expression (3). Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO™ Expression Vectors Provide:
• Topoisomerase I for one-step, 5-minute TOPO™ cloning PCR amplification of the gene of interest
• For high-level expression recombination CMV promoter for fluorescent fusion proteins
• Ability to fuse EmGFP or YFP to the N or C terminus of your protein
• Bsd resistance markers for rapid selection of stable cell lines
For Research Use Only. Not for use in diagnostic procedures.