Thermo Fisher L10119 200 assays LIVE/DEADFixable near-infrared dead cell stain kit for 633or 635nmexcitation
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L10119 200 assaysLIVE/DEAD Fixable Near-IR Dead Cell Stain Kit for 633 or 635 nm excitation

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Prior to fixation and permeabilization required for intracellular antibody staining, or prior to formaldehyde fixation to eliminate biohazardous materials, Determine cell viability using the LIVE/DEAD™ Fixable Near-Infrared Dead Cell Stain Kit. This kit is optimized and validated for use with red laser flow cytometers.

• Stable—dyes are freeze-dried in individual vials to maintain stability

• Robust—staining patterns are the same before and after fixation

• Bright signal—can be measured in single vials Channels easily distinguish live/dead cells

ViewA selection of all fixable viability dyes for flow cytometry analysis Guide.

Stable
Unlike products sold as solutions, LIVE/DEAD™ Fixable NIR Stain is conveniently packaged in 40-test vials to help ensure dye Stability and performance over time. Amine-reactive dyes in solution lose their effectiveness over a short period of time, so it is recommended that the vial be used up after rehydration. If this is not possible, aliquot the vial contents into small volumes and store at -80°C, avoiding freeze-thaw cycles.

Robust
Dead cell identification stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. LIVE/DEAD™ Fixable Near-IR Stain is an amine-reactive dye that covalently binds intracellular and extracellular amines, retaining the staining pattern after formaldehyde fixation.

Excellent brightness
LIVE/DEAD™ immobilizable near-infrared dyes were selected for use as identification dyes due to their fluorescent properties, i.e. when excited by a red laser Can send out bright signals. Near-infrared fluorescent reactive dyes have a maximum excitation wavelength of ∼633 nm, making them suitable for use with red or HeNe lasers that emit at ∼750 nm. Because the single-color dye and single channel of a flow cytometer can be used to distinguish between live and dead cells, it is ideal for multicolor experiments.

How it works
In cells with damaged cell membranes, the dye can react with free amine groups inside and on the cell surface to produce strong fluorescent staining. In living cells, the reactivity of the dye is limited to the amines on the cell surface, resulting in less fluorescence intensity. The intensity difference between live and dead cells is typically greater than 50-fold and can be easily distinguished.

Available in a variety of colors
LIVE/DEAD™ Fixable Dead Cell Stain is available in a variety of colors to meet your multicolor detection panel needs.
For Research Use Only. Not for use in diagnostic procedures.