LIVE/DEAD™ Fixable Blue Dead Cell Staining Kit can be used for fixation and permeabilization in intracellular antibody staining Determine cell viability before this step, or before fixation with formaldehyde to remove biohazardous materials. This kit is optimized and validated for use with UV laser flow cytometers.
•Stable—Dye is freeze-dried in individual vials to maintain stability
•Robust—Staining pattern is the same before and after fixation
•Bright signal—Ability to print in a single channel Easily distinguish live/dead cells
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Selection guide for all fixable viability dyes for flow cytometry analysis .
StableUnlike products sold as solutions, LIVE/DEAD™ Fixable Blue Stain is conveniently packaged in 40 test bottles to ensure long-lasting dye Maintain stability and performance over time. Amine-reactive dyes in solution lose their effectiveness over a short period of time, so it is recommended that the vial be used up after rehydration. If this is not possible, aliquot the vial contents into small volumes and store at -80°C, avoiding freeze-thaw cycles.
RobustDead cell identification stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. LIVE/DEAD™ Fixable Blue Stain is an amine-reactive dye that covalently binds intracellular and extracellular amines, retaining the staining pattern after formaldehyde fixation.
Excellent BrightnessThe LIVE/DEAD™ immobilizable blue dye was selected for its fluorescent properties to emit a bright signal when excited by a UV laser. The maximum excitation wavelength of blue fluorescent reactive dye is ∼350 nm, making it extremely suitable for use with UV lasers, and its emission wavelength is ∼450 nm. When performing cell viability staining, by turning off one channel of the blue 488 nm laser and switching to the UV laser, you can free up a channel on the flow cytometer for reagents that would be difficult to detect based on color alone without FITC or PE. When using a UV laser, there is no spectral overlap with other common dyes, so there is no need to compensate for the vitality stain with other stains in the kit. Because the single-color dye and single channel of a flow cytometer can be used to distinguish between live and dead cells, it is ideal for multicolor experiments.
How it worksIn cells with damaged cell membranes, the dye can react with free amine groups inside and on the cell surface to produce strong fluorescent staining. In living cells, the reactivity of the dye is limited to the amines on the cell surface, resulting in less fluorescence intensity. The intensity difference between live and dead cells is typically greater than 50-fold and can be easily distinguished.
Available in a variety of colorsLIVE/DEAD™ Fixable Dead Cell Stain is available in a variety of colors to meet your multicolor detection panel needs.
For Research Use Only. Not for use in diagnostic procedures.