LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit can be used for the fixation and permeabilization steps in intracellular antibody staining Determine cell viability before fixation with formaldehyde to remove biohazardous substances. This kit is optimized and validated for use with a purple laser flow cytometer.
• Stable—dyes are freeze-dried in individual vials to maintain stability
• Robust—staining patterns are the same before and after fixation
• Low compensation—other fluorophores There is minimal spectral overlap between
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A selection of all fixable viability dyes for flow cytometry analysis Guide.
StableUnlike products sold as solutions, LIVE/DEAD™ Fixable Yellow Stain is conveniently packaged in 40-test bottles to help ensure that the dye changes over time Go-ahead stability and performance. Amine-reactive dyes in solution lose their effectiveness over a short period of time, so it is recommended to use the vial completely after rehydration. If this is not possible, aliquot the vial contents into small volumes and store at -80°C, avoiding freeze-thaw cycles.
RobustDead cell identification stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. LIVE/DEAD™ Fixable Yellow Stain is an amine-reactive dye that covalently binds to intracellular and extracellular amines, and the staining pattern remains after formaldehyde fixation.
Low CompensationLIVE/DEAD™ Fixable Yellow Stain was selected for its fluorescent properties, i.e. it combines with other violet dyes and can be combined with 488 nm blue Minimizes the compensation required between laser-excited dyes. The maximum excitation wavelength of the yellow fluorescent reactive dye is approximately ∼405 nm, but it can be well excited by a violet laser at 405 nm. Its maximum emission wavelength is approximately ∼570 nm, allowing acquisition in the second or third channel of most violet laser flow cytometers.
How it worksFor cells with damaged cell membranes, the dye can interact with free amine groups inside the cells and on the cell surface to emit strong fluorescence. In living cells, the reactivity of the dye is limited to the amines on the cell surface, resulting in less fluorescence intensity. The intensity difference between live and dead cells is typically greater than 50-fold and can be easily distinguished.
Available in a variety of colorsLIVE/DEAD™ Fixable Dead Cell Stain is available in a variety of colors to meet your multicolor detection panel needs.
For Research Use Only. Not for use in diagnostic procedures.