Prior to fixation and permeabilization required for intracellular antibody staining, or prior to formaldehyde fixation to eliminate biohazardous materials, Determine cell viability using the LIVE/DEAD™ Fixable Green Dead Cell Stain Kit. This kit is optimized and validated for use with blue laser flow cytometers.

• Stable—Dye is freeze-dried in individual vials to maintain stability

• Robust—Staining pattern is the same before and after fixation

• Bright signal—Ability to print in single channel Easily distinguish live/dead cells

ViewSelection guide for all fixable viability dyes for flow cytometry analysis .

Stable
Unlike products sold as solutions, LIVE/DEAD™ Fixable Green Stain is conveniently packaged in 40-test bottles to help ensure that the dye remains stable over time Go-ahead stability and performance. Amine-reactive dyes in solution lose their effectiveness over a short period of time, so it is recommended to use the vial completely after rehydration. If this is not possible, aliquot the vial in small volumes and store at -80°C, avoiding freeze-thaw cycles.

Robust
Dead cell identification stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. LIVE/DEAD™ Fixable Green Stain is an amine-reactive dye that covalently binds intracellular and extracellular amines, retaining the staining pattern after formaldehyde fixation.

Excellent Brightness
LIVE/DEAD™ Fixable Green Stain was selected based on its fluorescent properties that provide a bright signal when excited with a blue 488 nm laser. The green fluorescent reactive dye has a maximum excitation wavelength of ∼495 nm, making it suitable for use with blue lasers, and an emission wavelength of ∼520 nm for acquisition in the FITC channel. Because the single-color dye and single channel of a flow cytometer can be used to distinguish between live and dead cells, it is ideal for multicolor experiments.

How it works
In cells with damaged cell membranes, the dye can react with free amine groups inside and on the cell surface to produce strong fluorescent staining. In living cells, the reactivity of the dye is limited to the amines on the cell surface, resulting in less fluorescence intensity. The intensity difference between live and dead cells is typically greater than 50-fold and can be easily distinguished.

Available in a variety of colors
LIVE/DEAD™ Fixable Dead Cell Stain is available in a variety of colors to meet your multicolor detection panel needs.