Thermo Fisher V13242 1 kitContains annexin VFITCand PIDead Cell Apoptosis Kit for Flow Cytometry Analysis
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V13242 1 kit Dead cell apoptosis kit containing Annexin V FITC and PI for flow cytometry analysis

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There are several advantages when measuring cell viability using the Annexin V-containing Dead Cell Apoptosis Kit for Flow Cytometry:

Excellent brightness
With protein Unlike other Annexin V kits that have lower concentrations or purification levels, Annexin V Alexa Fluor 488 Conjugate is optimized for flow cytometric analysis to show extreme differences between apoptotic and viable cells. Alexa Fluor 488 dye is an excellent green fluorescent dye with a spectrum similar to fluorescein (FITC).

High binding efficiency
Annexin V conjugates are made from high purity cys-annexin, which results in higher binding efficiency and enables accurate characterization of the apoptotic process Extremely high.

Multiparameter
Many publications require at least two different methods to identify apoptotic cells. This multi-parameter kit detects phosphatidylserine (PS) on the cytoplasmic surface of cell membranes while using propidium iodide to determine membrane integrity.

Apoptosis kit containing Annexin V Alexa Fluor 488 and PI
Apoptosis kit containing Annexin V Alexa Fluor 488 and propidium iodide (PI) The kit (V13241, V13245) is used to perform flow cytometry analysis to measure early apoptosis by detecting phosphatidylserine (PS) expression and membrane permeability. When cells were stained with Annexin V and propidium iodide, apoptotic cells expressing PS showed green fluorescence, which was detectable in the FITC channel, and showed low red fluorescence. Dead or necrotic cells show bright red fluorescence and no green fluorescence, while living cells show no green or red fluorescence.

The Dead Cell Apoptosis Kit containing Annexin V PE and SYTOX Green
The Dead Cell Apoptosis Kit containing Annexin V PE and SYTOX Green can be used for flow cytometry analysis During this period, recombinant annexin V coupled to orange fluorescent phycobiliprotein R-PE was used to detect PS externalization of apoptotic cells, and dead cells were detected using SYTOX Green nucleic acid stain. After treatment with the two probes, apoptotic cells showed orange fluorescence, dead cells showed green fluorescence, and living cells showed almost no fluorescence. These cell populations can be easily differentiated using the 530/30 nm and 585/42 nm bandpass filters of a 488 nm laser flow cytometer.

The Dead Cell Apoptosis Kit containing Annexin V APC and SYTOX Green
The Dead Cell Apoptosis Kit containing Annexin V APC and SYTOX Green can be used for flow cytometry analysis During this period, recombinant annexin V coupled to red laser excitation of allophycocyanin was used to detect PS externalization of apoptotic cells, and dead cells were detected using SYTOX Green nucleic acid stain. Detection of apoptotic cells by Annexin V binding to externalized PS. Viable cells show almost no fluorescence, apoptotic cells show red fluorescence and very little green fluorescence, and late apoptotic cells show higher levels of red and orange fluorescence. These cell populations can be easily distinguished by flow cytometry using 488 nm and 633 nm excitation sources (Argon ion laser and HeNe laser).

Dead Cell Apoptosis Kit Containing Annexin V FITC and PI
The Dead Cell Apoptosis Kit Containing Annexin V FITC and PI can use conjugated green fluorescent FITC dye Recombinant annexin V was used to detect PS externalization in apoptotic cells, and dead cells were detected using PI. Necrotic cells stained by PI show red fluorescence. After treatment with the two probes, apoptotic cells showed green fluorescence, dead cells showed red and green fluorescence, and living cells showed little or no fluorescence.

For Research Use Only. Not for use in diagnostic procedures.