Microbiological Culture Medium Xylose Lysine Deoxycholate Agar XLD agar tissue culture medium
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Purpose: For the selective isolation and cultivation of enteric pathogenic bacteria.
Composition (g/L):
Peptone: 20.0
Lactose: 10.0
Bile Salts: 5.0
Sodium Chloride: 5.0
Neutral Red: 0.075
Agar: 12.0
pH Value: 7.4 ± 0.2 at 25°C
Testing Principle:
Bile salts inhibit the growth of Gram-positive bacteria. Lactose fermentation is differentiated by the color change of neutral red, which distinguishes lactose-fermenting bacteria from non-lactose-fermenting bacteria. Shigella colonies appear colorless, translucent, smooth, moist, round, with edges that are either regular or irregular.
Usage:
Weigh 52.0g of this product, heat and dissolve it in 1000mL of distilled water. Sterilize by autoclaving at 121°C for 15 minutes, then store for later use.
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