Thermo Scientific GlycoLink Immobilization Kit 10 columns kit 88941

Features of the GlycoLink Immobilization Kit:

• High-capacity—immobilize 1 to 10 mg of oxidized antibody or other glycoprotein per milliliter of resin.

• Fast and efficient—immobilize in as few as 30 minutes; achieve at least 90% coupling of most glycoproteins in less than 4 hours (efficiency depends on the amount and type of glycosylation).

• Specific immobilization—the hydrazide-activated UltraLink Resin conjugates only to purified glycoproteins whose sugar (e.g., sialic acid) groups have been gently oxidized with periodate.

• Stable attachment—resonance structure of the hydrazone bonds are sufficiently stable for multiple affinity purifications with one batch of prepared resin; no stabilization step is required.

• Preserves antibody function—immobilizes IgG via the Fc region, thereby keeping both antigen binding sites available for capturing target.

The GlycoLink Kits use hydrazide-activated resin and a special catalyst to prepare affinity columns with polyclonal antibodies, which typically have abundant carbohydrates (glycosylation) on their Fc portions. Any glycosylated proteins (even monoclonal antibodies) that contain periodate-oxidizable carbohydrate groups can be immobilized with these kits to make reusable affinity columns. Antibodies immobilized by this hydrazide immobilization chemistry have unobstructed antigen-binding sites and optimal purification capability. Prepared columns with stable glycoproteins or antibodies can be regenerated and reused at least five times for affinity-purification without significant loss in binding capacity.

Applications:

• Purify antigens using covalently coupled antibodies

• Determine binding partners for a glycoprotein of interest

• Perform large-scale affinity purifications

If you have any more questions, please contact us for consultation.