Top brand Agrisera Goat anti-Rabbit IgG (H&L) HRP conjugated AS10668

Product information:

Immunogen: purified Rabbit IgG, whole molecule

Host: Goat

Clonality: Polyclonal 

Purity: Immunogen affinity purified goat IgG.

Format: Lyophilized

Quantity: 1 mg

Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.

For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Additional information:

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.

Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)

Application information:

Recommended dilution: 1:500 -1:5000 (ELISA), 1:500 -1:5000 (IHC), 1:500 -1:5000 (WB)

Confirmed reactivity: heavy chains of Rabbit IgG, Light chains on all Rabbit immunoglobulins

Predicted reactivity: Rabbit IgG Heavy and Light chains (H&L) of all Rabbit immunoglobulins

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional information:

Based on immunoelectrophoresis no reactivity is observed to:

non-immunoglobulin rabbit serum proteins

serum from bovine, human or mouse

IgG from human or mouse

If you have any more questions, please contact us for consultation.