YHLO original ACTH 2*50T/Kit C86035

The Human Adrenocorticotropic Hormone (ACTH) Immunohistochemistry Kit employs HRP-labelled streptavidin conjugate (HRP-SA) as its foundation, enabling detection of specific antigens within cells and tissues. 

This kit offers high sensitivity, strong specificity, accurate qualitative localisation, and clear background. 

Following binding of the primary antibody to its target antigen, a biotinylated secondary antibody specifically binds to the primary antibody. HRP-SA is then added to form an antigen-specific primary antibody-biotinylated secondary antibody-HRP-SA complex, which is visualised under a microscope.

Detection method: Chemiluminescent immunoassay

The kit uses a chemiluminescence immunoassay, usually a double-antibody sandwich method, with high sensitivity to detect ACTH concentrations as low as pg/mL, which is critical for clinical diagnosis.

Sample type: Human EDTA plasma 

Special note on sample requirements: ACTH is extremely unstable in plasma and is easily degraded by proteases. Venous blood must be collected using EDTA anticoagulant tubes, bathed in ice immediately after sampling, quickly centrifuged at 4°C to separate the plasma, and frozen at -20°C or below as soon as possible. Any processing delays may result in falsely lower results.

Core application: For the auxiliary diagnosis and differential diagnosis of hypothalamic-pituitary-adrenal axis disorders, such as Cushing's syndrome, Addison's disease, and pituitary adenomas.

Compatible Instruments: YH-LON series fully automated chemiluminescent immunoassay analysers (e.g., iFlash 3000 series)

If you have any more questions, please contact us for consultation.