Top brand Thermo Fisher PBR322 DNA SD0041

PBR322 is 4361 bp in length and contains:

• The rep replicon responsible for plasmid replication (origin – plasmid pMB1)

• The rop gene encoding the Rop protein, which promotes conversion of the unstable RNA I–RNA II complex into a stable complex and facilitates copy number reduction (source – plasmid pMB1)

• The bla gene encoding β-lactamase, conferring resistance to ampicillin (source – transposon Tn3)

• The tet gene encoding the tetracycline resistance protein (Source – plasmid pSC101)

• Circular sequences are numbered as follows: 1 denotes the first T of the specific EcoRI site GAATTC. Numbering increments sequentially through the tet gene, pMB1 material, and finally the Tn3 region.

Product Advantages:

• Isolated from Escherichia coli (dam+, dcm+)

• Over 90% supercoiled form

• Purified via chromatography using proprietary patented technology

Applications:

• Cloning

• Preparation of DNA molecular weight standards

 Specification:

If you have any more questions, please contact us for consultation.